Gap junction structures. IV. Asymmetric features revealed by low-irradiation microscopy.
نویسندگان
چکیده
منابع مشابه
Gap Junction Structures. IV. Revealed by Low-irradiation Asymmetric Microscopy Features
Micrographs of mouse liver gap junctions, isolated with detergents, and negatively stained with uranyl acetate, have been recorded by low-irradiation methods. Our Fourieraveraged micrographs of the hexagonal junct ion lattice show skewed, hexameric connexons with less stain at the threefold axis than at the six indentations between the lobes of the connexon image. These substructural features, ...
متن کاملGap junction structures. IV. Asymmetric features revealed by low- irradiation microscopy
Micrographs of mouse liver gap junctions, isolated with detergents, and negatively stained with uranyl acetate, have been recorded by low-irradiation methods. Our Fourier-averaged micrographs of the hexagonal junction lattice show skewed, hexameric connexons with less stain at the threefold axis than at the six indentations between the lobes of the connexon image. These substructural features, ...
متن کاملGap junction distribution in adult mammalian myocardium revealed by an anti-peptide antibody and laser scanning confocal microscopy.
A polyclonal antiserum, raised against a synthetic peptide matching part of the sequence of connexin43 (a rat cardiac gap-junctional protein), was used in combination with laser scanning confocal microscopy to investigate gap junction distribution in cardiac tissues from a range of mammalian species. Comparison of the localised punctate staining patterns obtained in ventricular tissue with the ...
متن کاملGap junction structures. I. Correlated electron microscopy and x-ray diffraction
X-ray crystallographic methods and electron microscope image analysis have been used to correlate the structure and the chemical composition of gap junction plaques isolated intact from mouse liver. The requirement that the interpretations of X-ray, electron microscope, and chemical measurements be consistent reduces the uncertainties inherent in the separate observations and leads to a unified...
متن کاملGap junction structure: unraveled, but not fully revealed
Gap junction channels facilitate the intercellular exchange of ions and small molecules, a process that is critical for the function of many different kinds of cells and tissues. Recent crystal structures of channels formed by one connexin isoform (connexin26) have been determined, and they have been subjected to molecular modeling. These studies have provided high-resolution models to gain ins...
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ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 1983
ISSN: 0021-9525,1540-8140
DOI: 10.1083/jcb.96.1.204